Data Independent Acquisition

What is DIA?

Conversely to strategies like SRM or PRM, Data Independent Acquisition, or DIA, requires no precursor information. Precursor ions are isolated in small, pre-determined isolation windows (usually 2-25m/z) and fragmented. The fragment ions of this isolation window enter the are scanned systematically across a range of m/zs (400m/z-1200m/z, for example).  Because no prior knowledge of peptides or proteins is required for isolation, this technique is suited for discovery-based proteomics or profiling an obscure proteome. 

DIA in a nutshell

infographic

Our workflow

infographic here

Cost breakdown

infographic + link to downloadable excel sheet

University of Washington

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