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University of Washington Genome Sciences Department
Data Independent Acquisition
What is DIA?
Conversely to strategies like SRM or PRM, Data Independent Acquisition, or DIA, requires no precursor information. Precursor ions are isolated in small, pre-determined isolation windows (usually 2-25m/z) and fragmented. The fragment ions of this isolation window enter the are scanned systematically across a range of m/zs (400m/z-1200m/z, for example). Because no prior knowledge of peptides or proteins is required for isolation, this technique is suited for discovery-based proteomics or profiling an obscure proteome.
DIA in a nutshell
infographic
Our workflow
infographic here
Cost breakdown
infographic + link to downloadable excel sheet
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